Fertility medications are prescribed to control the ova maturation and to increase the chance of collecting multiple ova during menstrual cycle referred to as ovulation induction. Multiple ova are desired because some of ova will not develop or fertilize after retrieval and ovum development is monitored by using ultrasound as well as urine or blood test samples are examined to check hormone levels
Follicular aspiration was done by retrieving ova are retrieved through a minor surgical procedure by using ultrasound imaging to guide a hollow needle through the pelvic cavity which removes ova from the ovaries.
Sperm, usually obtained by ejaculation is prepared for getting the fusion with the ova.
By insemination, the sperm and ova are placed in incubators located in the laboratory which enabled fertilization and in some cases, lower probability of fertilization was found, intracytoplasmic sperm injection (ICSI) may be done. The ova are monitored to confirm that fertilization and cell division are taking place and it was considered embryos after successful fertilization.
The embryos are usually transferred into the woman’s uterus from one to six days later, but in most cases the transfer occurs between two to three days following egg retrieval. At this stage, the fertilized egg has developed into a two-to-four cell embryo. The transfer process involves a speculum which is inserted into the vagina to expose the cervix. A predetermined number of embryos are suspended in fluid and gently placed through a catheter into the womb. These steps are followed sequentially under regular monitoring followed by all required blood tests and potential ultrasounds to determine successful implantation as well as finally desired occurrence of pregnancy.